Curriculum Vitae (CV)

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University of California – San Diego  Aug. 2020– Present

Ph.D. in Biological Sciences

University of Minnesota – Twin Cities (College of Biological Sciences)   Aug. 2013 – May. 2017 

Bachelor of Science in Biochemistry and Genetics, Cell Biology & Development 

Universidad de los Andes, Colombia   Jan. 2012 – May 2013

Department of Biological Sciences, Microbiology Undergraduate

Research Technician – Center for Engineering in Medicine    Aug. 2017 – Present

Harvard Medical School / Massachusetts General Hospital, Boston, MA. 

· Develop fluorescence-based reporter plasmids under the regulation of CYP450 drug metabolism

· Optimize culturing of primary human/rat/mouse hepatocytes in 2D, 3D and microfluidic platforms 

· Develop and validate NAFLD liver-on-a-chip model through marker staining and qRT-PCR

· Test drug toxicity and drug-drug interactions in vitro for microfluidic drug testing and discovery

· Host journal club and reviewed current scientific papers in a weekly basis 

· Train interns, technicians and post-docs in hepatocyte culturing, principles of microscopy and qRT-PCR

Undergraduate Teaching Assistant - Biochemistry Laboratory  Jan. 2016 – Aug. 2016

Biochemistry, Molecular Biology & Biophysics Dpt., University of Minnesota

· Graded laboratory reports on a weekly basis

· Assisted students on conceptual understanding of cloning, chromatography, Western Blot and electrophoresis. 

• B. Bulutoglu, C. Rey-Bedón, Y.B. Kang, S. Mert, M.L. Yarmush, O.B. Usta (2019) A microfluidic patterned model of non-alcoholic fatty liver disease: applications to disease progression and zonation. Lab-on-a-Chip. 19(18), 3022-3031. 
• B. Bulutogu, S. Mert., C. Rey-Bedón. et al. (2019). Rapid maturation of the hepatic cell line Huh7 via CDK inhibition for PXR dependent CYP450 metabolism and induction. . Nature - Sci. Rep. 15848 doi:10.1038/s41598-019-52174-w
• H. Huang, C. Rey-Bedón. et al. (2019). Deep-supercooling for extended preservation of adipose-derived stem cells. Cryobiology. j.cryobiol.2019.11.004
• B. Bulutoglu, C. Rey-Bedón. et al. (2019) A comprehensive comparison of hepato-cellular in vitro platforms to study CYP3A4 induction. PLoS ONE. Under Review.
• C. Rey-Bedón, P. D. Banik. et al.(2020). Determining drug inducibility of CYP450 in a NAFLD model with Primary Human Hepatocytes. In Submission.

Minnesota iGEM Member        May – Oct. 2014

Biotechnology Institute, University of Minnesota

· Performed gene cloning in Escherichia coli to develop a bacterial operon capable of remediating mercury

· Taught synthetic biology to the public through educational panels at the Science Museum of Minnesota

· Awarded for Best Environment Over-grad Team Project and a Gold Medal

Undergraduate HHMI Research Fellow     Jan. – May 2014

Gralnick Lab, Dr. Jeffrey Gralnick & Dr. James Parejko

Biotechnology Institute, University of Minnesota

· Awarded with HHMI stipend for undergraduate research

· Screened MATE bfe transporter excretion in various Vibrio spp. through bulk fluorescence assay and HPLC

· Cloned Vibrio parahaemolyticus bfe-transporter into Δbfe Shewanella oneidensis to test complementarity

· Developed phylogenetic tree of Shewanella and Vibrio spp. based on a bfe MSA through MEGA bioinformatics

· Presented results at HHMI Research Symposium

Undergraduate Research Assistant   Aug. 2012 – May 2013 Biophysics Lab, Dr. Juan Manuel Pedraza

Universidad de los Andes, Bogotá, Colombia

· Reviewed research papers on relevant biophysics topics for a weekly discussion with Dr. Pedraza

· Learned basic research laboratory techniques including microbial culture and bacterial persistence assessment  

· Planned persistence gene mapping experiment for E. coli based on partial chromosome conjugation 

Cell Resource Core (CRC) Member   Jan. 2018 - Present

Massachusetts General Hospital, Boston, MA. 

· Cryopreserve human primary hepatocytes from non-transplantable liver donors for non-profit vending 

· Optimize palatability and long-term culture media for cryopreserved hepatocytes

· Characterize hepatic phenotype and drug mediated CYP450 inducibility 

· Consult CRC clients for troubleshooting on hepatic cryopreservation, thawing and culturing 

MIT Grand Hack – Medicine 2019   May 2019

MIT Media Lab, Cambridge, MA

· Developed a project on commercialization of T-Cell Receptor sequencing data 

· Coded mockup ML algorithm for antigen T-Cell Receptor matching

· Presented the project before prominent researchers and entrepreneurs at MIT Media Lab 

Global Gopher Guide, International Student and Scholar Services   Aug. 2014

University of Minnesota-Twin Cities, Minneapolis, MN

· Toured incoming international students around the University’s campus and student-living facilities

· Organized a daily Tea meeting where Intl. students could connect with other new and current students

· Awarded with stipend by the Culture Corps Program

Vice President, Latino International Student Association       Sept. 2013 - May 2016 University of Minnesota-Twin Cities, Minneapolis, MN

· Planned different community activities to connect the Hispanic culture with the local Minnesotan students

· Assisted in treasury of the association through group discussions and connecting with other organizations

Laboratory skills: Molecular Cloning, Microfluidic Culturing, Mammalian 3D Micro Tissue Culturing, Human Hepatocyte Cryopreservation, Mammalian Cell Transfection, ELISA, PCR, qRT-PCR, RNA-Seq, Luminex Assays, Primer Design, SDS-Page, Western Blot, Gel Electrophoresis, Duoflow Chromatography, HPLC, Microbial Culturing, Transfection and Conjugation.

Foreign Languages: Spanish (Native Language), English (Advanced), German (Advanced)

Computer Skills: R, FIJI, Microsoft Office Word, Prism, ImageJ macros, NCBI Blasts, MEGA, and Python.

Hobbies and interests: Classical guitar, assistive technologies, astrophysics, soccer and multicultural activities.